Okolie Charles Emeka Profile

DOCTOR OKOLIE CHARLES EMEKA


Department:
Biological Sciences

Email:
okolie.charles@lmu.edu.ng

« Born and brought up in Nigeria. Had additional education in the United Kingdom. Molecular Microbiologist, maintaining high standards of Biological Safety and Ethics

« Enthusiastic for original research and problem-solving innovations especially in molecular diagnostics (MDx)

« Highly skilled in the design, development, validation, and implementation of PCR and real-time PCR assays for accurate and rapid identification of microbes in samples from humans, animals, crops/plants and the environment (earth, cavity ground water, and crude oil)

« Working independently as well as closely within multi-disciplinary team and matrix management environment

Okolie CE et al (2015). Development of a new pentaplex real-time PCR assay for the identification of poly-microbial specimens containing Staphylococcus aureus and other staphylococci with simultaneous detection of staphylococcal virulence and methicillin resistance markers. Mol Cell Probes 29(3):144-150. DOI: PDF.
Okolie CE et al (2015). Development of a heptaplex PCR assay for identification of Staphylococcus aureus and CoNS with simultaneous detection of virulence and antibiotic resistance genes. BMC Microbiology. 15:157. PDF.

C. E. Okolie, U. C. Essien and J. Idoko (2015). Genetic and phenotypic identification of vancomycin-resistant Staphylococcus aureus isolates from retail poultry carcasses in Omu-Aran, North-Central Nigeria. British Biotechnology Journal, 6(2):87-92. PDF.
Charles Emeka Okolie and Richard James (2015). Optimization And Evaluation Of Triplex Real-Time PCR Assay For Detection Of Genes Encoding Staphylococcal Virulence and Methicillin Resistance Using Two Different Multi-Channel Emission Instruments. Journal of Applied Life Sciences International, 2(4):145-151. PDF. 
Charles Emeka Okolie and Richard James (2015). Development Of New Pentaplex PCR Assay For Differentiating Staphylococci From Other Bacteria With Simultaneous Detection Of Staphylococcus aureus Genes Encoding Panton-Valentine Leukocidin and Methicillin Resistance. Journal of Advances in Biology and Biotechnology, 2(4): 250-259. PDF.
Charles E. Okolie, Alan Cockayne, Karl Wooldridge, Richard James (2014). Development and Validation of a New Diagnostic PCR Assay for vanA Gene Encoding Vancomycin Resistance in Staphylococcus aureus. International Journal of Applied Microbiology and Biotechnology Research; 2:1-10. PDF. 
Emmanuel Nna, Jonathan Madukwe, Ejike Egbujo, Chris Obiorah, Charles Okolie, Godwin Echejoh, Amina Yahaya, James Adisa, and Ijeoma Uzoma (2013). Gene Expression of Aurora Kinases in Prostate Cancer and Nodular Hyperplasia Tissues. Med Princ Pract.; 22(2):138-43.
Adaobi I Ekwempu, Charles E Okolie, Essien U Cosmas, Gwom I Davou, John Shindang, James O Adisa (2013) Evaluation of the research writing skills of Medical Laboratory Scientists in Nigeria. Journal of Medical Research and Practice; 2(1):34-37 (PDF).
Mary-Jane N. Ofojekwu, Ogbonnaya U. Nnanna, Charles E. Okolie, Lolade A. Odewumi, Ikechukwu O. U. Isiguzoro, and Moses. D. Lugos (2013) Hemoglobin and Serum Iron Concentrations in Menstruating Nulliparous Women in Jos, Nigeria. Lab Med: 44:121-124.
U.C. Essien, C.C. Iheukwumere, G.I. Davou, Z. Sheyin, C.E. Okolie, F.R. Ede and A.I. Ekwempu (2015). Prevalence and Predictors of Asymptomatic Urinary Tract Infection among HIV Positive Patients in Jos, North Central Nigeria. IJCMAS, 4(9): 454-462. PDF.
O. B. Akpor, T. D. Olaolu and E.C. Okolie (2013). The Effect of Temperature on Nitrate and Phosphate Uptake from Synthetic Wastewater by Selected Bacteria Species. British Microbiology Research Journal; 4(3)328-342. PDF.
Okolie CE, Cockayne A, Penfold C, James R (201

Okolie CE, Wooldridge KG, Turner DP, Cockayne A, James R (2015). Development of a new pentaplex real-time PCR assay for the identification of poly-microbial specimens containing Staphylococcus aureus and other staphylococci with simultaneous detection of staphylococcal virulence and methicillin resistance markers. Mol Cell Probes 29(3):144-150. DOI: PDF.
Okolie CE, Wooldridge KG, Turner DP, Cockayne A, James R (2015). Development of a heptaplex PCR assay for identification of Staphylococcus aureus and CoNS with simultaneous detection of virulence and antibiotic resistance genes. BMC Microbiology. 15:157. PDF.
Emmanuel Nna, Jonathan Madukwe, Ejike Egbujo, Chris Obiorah, Charles Okolie, Godwin Echejoh, Amina Yahaya, James Adisa, and Ijeoma Uzoma (2013). Gene Expression of Aurora Kinases in Prostate Cancer and Nodular Hyperplasia Tissues. Med Princ Pract.; 22(2):138-43.
Okolie CE, Cockayne A, Penfold C, James R (2013). Engineering of the LukS-PV and LukF-PV subunits of Staphylococcus aureus Panton-Valentine leukocidin for Diagnostic and Therapeutic Applications. BMC Biotechnol.;13(1):103. PDF.

 

· BCH211: Biomolecules I

· MCB211: Introductory General Microbiology

· MCB221: Introductory Microbial Ecology

· BCH224: Introductory Molecular Biology

· MCB312: Microbial Physiology

· BCH313: Metabolism of Amino Acids and Proteins

· BCH316: Metabolism of Nucleic Acids

· MCB317: Immunology

· BCH417: Biochemistry of Macromolecules

· MCB415: Microbial Genetics and Molecular Biology

· MCB421: Antimicrobial Agents

· MCB422: Industrial Microbiology

Certificate in Food Safety and Hygiene, Institution for Occupational Health and safety (UK and Ireland), 2004. 

Fellow in Virology, Medical Laboratory Council of Nigeria (since 2001).

Associate in Bacteriology, Medical Laboratory Council of Nigeria (since 1992).

 

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